AIM:To evaluate the effect of stereoscopic 3D/4D technology as a visual training system combined with refractive correction and masking therapy for adult amblyopia.METHODS: A retrospective study. From July 2018 to October 2020, totally 41 cases(57 eyes)adult amblyopia in our hospital were divided into severe, moderate, mild group, effect of stereoscopic 3D/4D technology training were analyzed after 6mo, including the best corrected visual acuity, visual evoked potentials, stereoscopic function.RESULTS: After treatment, best corrected visual acuity of the patients were significantly improved, the effective rate of severe, moderate, mild amblyopia group were 69%, 88%, 75%, the total effective rate was 79%. The stereoscopic function of the patients were significantly improved, the effective rate of severe, moderate, mild amblyopia group were 62%, 75%, 80%, the total effective rate was 74%. The P100 wave latency periods of the visual evoked potentials were shortened after 6mo treatment, and the P100 wave amplitudes were increased(P<0.05). CONCLUSION:Stereoscopic 3D/4D technology training combined with refractive correction and masking therapy can effectively treat the adult amblyopic.