Microbial mixed infectious keratitis is an ocular surface disease caused by corneal infection, which has an acute onset and rapid progression and can lead to blindness in severe cases. Establishing an animal model of microbial mixed infectious keratitis is conducive to exploring its pathogenesis, prevention, clinical diagnosis and treatment. This article reviews the methods of making animal models of mixed infectious keratitis with microorganisms and the diagnostic methods after successful modelling infections, aiming to provide references for the further development and research of animal models of the disease.