目的：探讨眼部蠕形螨感染影响睑酯菌群组成的相关分析。

方法：采用非干预性、观察性研究的方法，选取2020-07/2021-02我院39例受试者，根据有无蠕形螨感染或有无睑板腺功能障碍(MGD)将受试者分为三组，对照组(14例)、睑板腺功能障碍组(MGD组，14组)、蠕形螨感染组(FM组，11组)。对三组受试者睑板腺睑酯样本进行16S rRNA基因V3～V4片段的高通量测序，测序数据进行生物信息学分析，以研究眼部蠕形螨受试者睑酯菌群的构成及差异。

结果：蠕形螨感染组的假单胞菌属和丛毛单胞菌属丰度均显著高于健康对照组和MGD组(P<0.05)，罗尔斯通氏菌属丰度显著低于对照组和MGD组(P<0.05)。MGD组和FM组睑酯菌群微生物丰富度、微生物群落多样性显著高于健康对照组(P<0.05)。

结论：蠕形螨感染改变了睑酯菌群的构成，并提高了睑酯菌群的微生物丰富度和群落多样性。

METHODS: A non-interventional and observational study was performed on recruited 39 patients in our hospital between July 2020 and February 2021. They were divided into control group(n=14), meibomian gland dysfunction(MGD)group(n=14), and demodex group(FM, n=11)according to the presence or absence of demodex infection or MGD. High-throughput sequencing of V3-V4 fragment of 16S rRNA gene was performed on the meibomian ester samples of the three groups of subjects, and bioinformatics analysis was performed on the sequencing data to study the composition and difference of meibum lipid flora in the subjects of ocular demodex.

RESULTS: Pseudomonas and Comamonas in FM group were significantly higher than those in control group and MGD group(P<0.05), while Ralstonia in Demodex infection group was significantly lower than that in control group and MGD group(P<0.05). The microbial richness and community diversity of meibum lipid flora of the MGD group and the FM group were significantly higher than those of the control group(P<0.05).

CONCLUSION: Ocular demodex infection changed the composition of meibum lipid flora and increased the microbial richness and community diversity of meibum lipid flora.