[关键词]
[摘要]
[已撤稿] 目的：观察六味地黄汤对老化模型大鼠视网膜组织中铁蛋白、溶质载体家族7成员11(SLC7A11)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶4(GPX4)表达水平的影响。
方法：将45只SD大鼠随机分成空白组、模型组、中药组，各15只。空白组腹腔注射生理盐水，模型组、中药组腹腔注射D-半乳糖\〖500 mg/(kg·d)\〗，同时，中药组灌服六味地黄汤\〖6.75 g/(kg·d)\〗，空白组、模型组灌服等体积生理盐水，连续8 wk。检测各组大鼠视网膜组织中铁蛋白、SLC7A11、GSH、GPX4的表达水平。
结果：模型组较空白组大鼠视网膜组织中铁蛋白表达增高(P<0.05)，GSH、SLC7A11、GPX4表达降低(P<0.05)。中药组较模型组大鼠视网膜组织中GSH、SLC7A11、GPX4表达增高(P<0.05)。
结论：六味地黄汤可能通过调控铁死亡途径发挥延缓视网膜老化的作用。
[Key word]
[Abstract]
AIM: To observe the effects of Liuwei Dihuang Tang on the expression levels of ferritin, recombinant solute carrier family 7 member 11(SLC7A11), glutathione(GSH), and glutathione peroxidase 4(GPX4)in retinal of aging model rats.
METHODS: A total of 45 SD rats were randomly divided into a blank group, a model group, and a traditional Chinese medicine(TCM)group, with 15 rats in each group. The blank group was intraperitoneally injected with physiological saline, while the model group and TCM group were intraperitoneally injected with D-galactose \〖500 mg/(kg·d)\〗. At the same time, the TCM group was orally administered with Liuwei Dihuang Tang \〖6.75 g/(kg·d)\〗, while the blank group and model group were orally administered with equal volume of physiological saline for 8 consecutive wk. The expression levels of ferritin, SLC7A11, GSH, and GPX4 in the retinal tissues of rats in each group were detected.
RESULTS: The expression of ferritin in the retinal tissue of the model group was increased compared to the blank group(P<0.05), while the expression of GSH, SLC7A11, and GPX4 was reduced(P<0.05). The expression of GSH, SLC7A11, and GPX4 in the retina tissue of rats in the TCM group was higher than that in the model group(P<0.05).
CONCLUSIONS: Liuwei Dihuang Tang may exert a delaying effect on retinal aging by regulating the ferroptosis pathway.
[中图分类号]
[基金项目]
国家自然科学基金青年科学基金项目(No.82104714); 国家中医药管理局“中医药循证能力建设项目”(No.2019XZZX-YK008)
