[关键词]
[摘要]
目的：观察通过改进方法制备出的新型功能化上转换纳米材料UCNPs-PEG-Tf对脉络膜黑色素瘤细胞(OCM-1)的特异性成像现象。
方法：制备出NaYF4：Yb/Er(UCNPs)，再等离子体处理将之表面羧基化； 分别接载氨基聚乙二醇和转铁蛋白(Tf)制备出UCNPs-PEG-Tf。对其进行相应表征，检测其生物相容性，通过荧光分光光度仪和倒置荧光显微镜检测对OCM-1的特异性荧光现象。
结果：相应表征证明成功合成了UCNPs和UCNPs-PEG，以及进一步接载Tf后形成的UCNPs-PEG-Tf。复合物UCNPs-PEG-Tf具有卓越的生物相容性，并且能发出显著的绿色荧光。同样条件下，荧光强度检测结果和倒置荧光显微镜的观察结果均证明其在细胞OCM-1成像方面的显著特异性。
结论：合成的新型功能化UCNPs-PEG-Tf纳米复合物生物相容性好，且可以对细胞OCM-1特异性成像。
[Key word]
[Abstract]
AIM: To prepare a novel functionalized upconversion nanomaterial UCNPs-PEG-Tf through an improved method and observe its specific imaging phenomenon to choroidal melanoma cells(OCM-1).
METHODS: NaYF4:Yb/Er(Up-conversion nanoparticles, UCNPs)was Prepared and plasma was treated to carboxylate the surface; then amino polyethylene glycol and transferrin(Tf)were respectively loaded to prepare UCNPs-PEG-Tf. Characterized them accordingly, its biocompatibility was tested accordingly, and the specific fluorescence phenomenon of OCM-1 was detected by fluorescence spectrophotometer and inverted fluorescence microscopy.
RESULTS: Characterization confirmed successful synthesis of UCNPs, UCNPs-PEG, and further loading of Tf to form UCNPs-PEG-Tf. UCNPs-PEG-Tf showed excellent biocompatibility and emitted significant green fluorescence. Under the same conditions, fluorescence intensity measurement and observations from the inverted fluorescence microscope both demonstrated its significant specificity in imaging to OCM-1 cells.
CONCLUSION: The synthesized novel functionalized UCNPs-PEG-Tf nanocomposite showed good biocompatibility and achieve specific imaging to OCM-1 cells.
[中图分类号]
[基金项目]
河南省医学科技攻关计划(联合共建)项目(No.LHGJ20190289)
