AIM:To investigate influence used times of microke-ratome blades on corneal flap quality (including corneal flap thickness,uniformity of thickness and smoothness of cut surface) in LASIK through imitating the course that corneal flaps were made on freshly enucleated swine eyes.·METHODS:Thirty-six freshly enucleated swine eyes were divided into 6 groups(Ⅰ-Ⅵ) according to the times blades were used(1-6).Ⅰand Ⅱ,Ⅲ and Ⅳ,Ⅴ and Ⅵ were combined separately to A,B,C groups.Central corneal thickness,corneal refractive power and IOP were measured.The course that corneal flaps were made was imitated on enucleated swine eyes and central corneal flap thickness was calculated.3 flaps from every groups wew chosen and uniformity of corneal flap thickness was observed with optical microscopy.1 flap from three groups were chosen (Ⅰ,Ⅳ,Ⅳ)and smoothness of cut surface was observed with scanning electron microscopy.·RESULTS:There was no difference between the 6 groups in central corneal thickness、corneal refractive power and IOP before operation.The everage central corneal flap thickness of Ⅰ-Ⅵ group were 152.7±14.6,143.8±9.3,128.3±12.7,114.2±9.6,99.7±5.7,70.0±12.7μm.There was no difference between Ⅰand Ⅱ,but Ⅲ-Ⅵ were different from Ⅰin central corneal flap thickness.There was a negative correlation between corneal flap thickness and the used times of blades.The average corneal flap thickness of A-C were 150.8±15.4,121.2±13.0,88.3±15.1μm.There was significant difference between the two groups.With the increase in the used times of blades,uniformity of corneal flap thickness decreased with optical microscopy and smoothness of cut surface decreased with scanning electron microscopy.·CONCLUSION:With the increase in the used times of blades,corneal flap thickness became thinner,uniformity of corneal flap thickness and smoothness of cut surface decreased.