·AIM:To learn the up-regulation of aquaporin protein 3(AQP3)expression in the corneal epithelial healing process of mice by cyclosporin A(CsA)eye drops.·METHODS:Models were established in the mechanical curettage method of corneal epithelium in mice to observe the repair of corneal epithelium.Corneas were treated with AQP3 immunohistochemical staining to observe the expression of AQP3.·RESULTS:AQP3 expression could be seen in the basal cell layer of the cornea.After applications of 125,250,500,1000mg/L CsA eye drops,the AQP3 expression was increased progressively.When corneal epithelium injury was treated with 500mg/L CsA eye drops,there was no statistical difference in healing size between the experimental group and control group 6 hours after injury,while 12,18,24 hours after injury there was a statistically significant difference(P<0.05).·CONCLUSION:500mg/L CsA eye drops can accelerate mice corneal epithelial damage repair by increasing the expression of AQP3.·