·AIM:To observe the clinical effect of the retrograde rip of pterygium combined with in situ autograft conjunctival flap rotational transplantation in the treatment of pterygium.·METHODS:Under a microscope,retrograde rip and removal of pterygium was performed,a 5mm×2mm conjunctival flap was cut off,after in situ 360° rotation,it was closely stitched to limbal and nasal bulbar conjunctiva.Postoperative follow-up was 3 to 18 months.·RESULTS:The grafts survived well in all cases with only four cases of recurrence in 64 cases.·CONCLUSION:Retrograde rip of pterygium combined with in situ autograft conjunctival flap rotational transplantation can reduce the local inflammation,change the limbus shift,the promotion of corneal limbal stem cells in micro-environment and make the repair of corneal limbal stem cells into corneal epithelial cell differentiation,is currently the treatment of pterygium and effective methods.·