AIM:To observe the change of mucus MUC5AC (Mucin5AC) selected by goblet cell in normal subjects and in soft contact lens (SCL) wear subjects. ·METHODS: Conjunctival impression cytology(CIC) were used to collect goblet cell, immunohistochemistry was used to detect the express level of MUC5AC selected by golbelt cell in normal subjects and in SCL wear subjects.·RESULTS: A significant decrease of MUC5AC was observed in the long-term(>5 years) and short-term(<5 years)SCL group compared with the control group (P1<0.01; P2<0.01),but analysis of the two SCL groups had no significant difference.·CONCLUSION: This study confirms difference in expression of the mucin MUC5AC in SCL and the health adult. It demonstrates a significant increase of dry eye symptom in SCL compared with the normals.