AIM:To measure the concentration of connective tissue growth factor(CTGF) and stromal cell-derived factor-1(SDF-1) in vitreous and to observe its relationship with diabetic retinopathy(DR) formation.METHODS:By enzyme linked immunosorbent assay(ELISA) method,the concentration of SDF-1 and CTGF in vitreous in 33 cases of proliferative diabetic retinopathy (PDR),5 cases of background diabetic retinopathy(BDR)and 5 cases of normal control were measured.RESULTS:CTGF concentration in vitreous of PDR patients was significantly increased compared with that in normal group(P<0.01),BDR patients(P<0.01).SDF-1 level in vitreous of PDR compared with BDR was significantly increased (P<0.05).CONCLUSION:SDF-1 and CTGF may play certain role in the development of PDR.