目的:检测一氧化氮(nitric oxide,NO)在缺氧性视网膜病变患者的玻璃体和血中的浓度变化,并与血管内皮生长因子(vascular endothelial growth factor,VEGF)相比较,从而阐明两者的关系。方法:增生性糖尿病视网膜病变(proliferative diabetic reti-nopathy,PDR)16例,视网膜分支静脉阻塞5例,孔源性视网膜脱离11例和黄斑裂孔10例。抽取患者血液,并于玻璃体切除手术中抽取玻璃体。Griess反应检测NO的浓度,ELISA检测VEGF的浓度。结果:玻璃体中NO浓度在PDR组中明显升高(15.2,4.6~50.9μmol/L),与其他3组相比具有统计学意义(P=0.0046);血液中的NO浓度在4组中无明显差异。玻璃体中的VEGF浓度在PDR(1507.2,50.7~3722.0mg/L)和BRVO(838.8,212.7~850.6mg/L)组中均明显升高,与其他两组相比具有统计学意义(P=0.0008),而PDR组又高于BRVO组(P=0.001);各组病例血液中VEGF浓度无明显差异(P=0.76)。4组病例中,NO和VEGF间均未见相关性。结论:NO和VEGF在PDR组的玻璃体中均明显升高,表明二者参与了糖尿病视网膜新生血管生成的过程。

·AIM:To evaluate the expressions of nitric oxide(NO)and VEGF in vitreous humor and blood samples in patients with proliferative diabetic retinopathy(PDR)and in patients with branch retinal vein occlusion(BRVO)and to compare the levels with those of controls.·METHODS:NO concentrations were determined using the Greiss reaction in plasma and vitreous humor sam-ples.VEGF levels were assayed by ELISA.The patients in the studies were divided into four groups:16 patients with PDR,5 patients with BRVO,11 patients with rhegmato-genous retinal detachment and 10 patients with macular hole.·RESULTS:The vitreous fluid levels of NO were signifi-cantly higher in patients with PDR than those in other three groups(15.2,4.6-50.9μmol/L,all P<0.01).The con-centration of VEGF were significantly higher in patients with PDR and BRVO than other two groups(P=0.0008,1507.2,50.7-3722.0mg/L;838.8,212.7-850.6mg/L respec-tively),but highest in PDR(P=0.001).There was no significance with NO and VEGF in serum in four groups.There was no correlation with that of NO and VEGF in four groups whatever in vitreous or plasma.·CONCLUSION:The results suggest that higher levels of NO and VEGF may be related to the angiogenesis in DR.·

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中国上海市重点学科建设项目(No.S30205);中国上海市科委基础重点项目(No.08JC1415600);中国上海市卫生局基金项目(No.2007013)~~