·AIM:To observe the changes of tear film functions in trabeculectomy by using human amniotic membrane(HAM)or mitomycin C(MMC).·METHODS:In a randomized control clinical trial,44 eyes 36 patients were divided randomly into two groups,and each group had 22 eyes.The former was underwent trabeculectomy with HAM transplanted and adjustable suture line,and the later was administrated intra-operatively with MMC and adjustable suture line.Slit-lamp microscope examination,cornea fluorescence integral(FI)staining,tear break-up time(BUT)and Schirmer Ⅰ test(SⅠt)were carried out 1 day before,1 month and 3 months after operation in the 44 eyes.·RESULTS:Compared with the data before operation,all of the cornea FI scores,SⅠt and BUT were statistically significant 1 month after operation(P<0.05),but there was no statistical significance between the two groups.3 months after operation,all of the data returned to their preoperative values in HAM group,but the tear film functions were significantly decreased in MMC group,there was statistical significance between the two groups(P<0.05).·CONCLUSION:The changes of tear film functions in the combined trabeculectomy with HAM and adjustable suture line are smaller than those in the MMC group.It is a safe and effective method for treatment of glaucoma.·