AIM:To investigate the effect of flavone on oxidation-induced injury in retinal pigment epithelium(RPE) cells. ·METHODS:In in vivo studies,NaIO3-induced RPE degeneration in rat eyes were treated with 5g/L flavone eye drops 3 times a day for 1 week before and 4 weeks after NaIO3 injection. At the end of 2 and 4 weeks,all rats were measured c-wave by electroretinogram(ERG). In in vitro studies,ARPE-19 cells were treated with hypoxia,H2O2,NaN3 and t-BHP to induce cell damages. MTT assay was used to measure the viable cells. ·RESULTS:The ERG c-wave results showed that flavone reversed NaIO3-induced injury at the end of 4 weeks. In vitro results showed flavone reversed the various oxidants-induced injuries in RPE cells. ·CONCLUSION:Flavone could prevent the RPE from oxidation-induced injury both in vivo and in vitro.