AIM:To understand the effect of Chitosan gelatin absorbable stent on alleviating the rabbits eyes of filtering bleb scarring. ·METHODS:Preparation of Chitosan gelatin absorbable membrane as implants were applied in rabbit eye filtration surgery under the conjunctiva ,and the control group was established. The postoperative filtering bleb condition was observed by slit-lamp. Eyes were removed to stain with HE on postoperative day 7 and 14,and the percentage of the filtering bleb area in the conjunctiva was calculated,and the effect of Chitosan gelatin absorbable membrane was analyzed according to the filtering bleb formation and fibrosis by independent-samples t test. ·RESULTS:In the postoperative day 7,the bulging diffuse bleb could be seen in both groups. In the postoperative day 14,flat bleb could be seen in the experimental group and no bleb but a large number of fibrous tissue in the control group. The percentage of the filtering bleb area in the conjunctiva were 6.16%±1.02%,5.96%±0.85% in the experimental and control group,respectively in the postoperative day 7 and there was no statistical difference.However,in the postoperative day 14,The percentage of the filtering bleb area in the conjunctiva was 1.09%±0.13% in the experimental group,but no filtering bleb in the control. The percentage was higher in the experimental group than in the control group by the independent-samples t test (t=8.52 and P=0.012). ·CONCLUSION:It is effective to prevent filtering bleb scarring with the Chitosan gelatin absorbable membrane.