AIM:To observe and investigate the method and clinical effectiveness of using the N-Octyl-Cyanoacrylate/N-Butyl-Cyanoacryl-ate(N-OCA/N-BCA) on sticking scleral flap in trabeculectomy. ·METHODS:We chose prospective-clinical-contrast study,randomly devided those 40 patients who received glaucoma trabeculectomy into 2 groups:20 patients 25 eyes in the bonding group using cyanoacrylate to stick scleral flap,20 patients 28 eyes in the sutures group using 10-0 nylon line to suture scleral flap. We evaluated the benefits of them with respect to surgical effects,responses,complications,intraocular pressure(IOP),vision,field of vision and so on. ·RESULTS:After following up for 6-24 months,compared the bonding group(25 eyes of 20 cases) with the sutures group(28 eyes of 20 cases),the difference of the postoperative IOP was not statistically significant(P>0.05); After surgery,in bonding group,3 eyes’ (1 eye on two weeks later,2 eyes on one month later) IOP >21mmHg,through local drugs therapy,it fell down to normal level; in sutures group,2 eyes’(7 days later) IOP >21mmHg,through local drugs therapy,1 eye became normal,but another eye failed in this way and took the second surgery six months later; The eyes in two groups mainly tended to form type Ⅱ blebs according to Kronfeld classification,the functional filtration bleb was 84% and 82% in bonding group and sutures group,respectively; Complications in both groups were few; Vision and field of vision almost had no change after surgery,without statistical significance. ·CONCLUSION:The using of N-OCA/N-BCA instead of lines to stick scleral flap in trabeculectomy makes the surgery safer and easier,and can surpass sutures to some extent.It is worth spreading and studying for clinical use.