AIM:To explore the frequency of drug injection of alloxan diabetes on the established model of rabbit. METHODS:Thirty-six healthy rabbits,weighing 2- 2.5kg,were randomly divided into one time drug injection group(group A,n = 12),two times drug injection group (group B,n = 12) and three times drug injection group (group C,n = 12).Each rabbit was injected with a total amount of 150mg/kg of alloxan.Fasting blood glucose was measured.The success rate and death rate of each group were also calculated. RESULTS:The success rate of diabetic rabbit model in group B was higher than that in group A(P<0.01) and its death rate was lower than that of group A(P<0.01);the success rate of diabetic rabbit model in group C was highest and the death rate was the lowest in three groups (P<0.01). CONCLUSION:Multiple administration of alloxan can improve success rate in establishing diabetic rabbit model with decreased death rate and increased stability.