AIM:To investigate the inhibiting effect of intravitreal defibrase and vancomycin injection on proliferative vitreoretinopathy (PVR), infectious endophthalmitis in rabbit model eyes of hemorrhagic penetrating ocular injury.
METHODS: Totally 40 New Zealand albino rabbits (right eyes) were taken as experimental rabbit model eyes of hemorrhagic penetrating ocular injury and all their left eyes were intact. The rabbits were divided into four groups randomly: phosphate buffered saline group（10 eyes）which were injected with 0.1mL phosphate buffered saline in vitreous; defibrase group（10 eyes）which were injected with 0.1U（0.1mL）defibrase in vitreous; vancomycin group（10 eyes）which were injected with 1mg （0.1mL）vancomycin in vitreous; defibrase combined with vancomycin group（10 eyes）which were injected with 01U（0.1mL）defibrase and 1mg （0.1mL）vancomycin in vitreous respectively. The inflammation in anterior segment of eye was estimated by slit-lamp microscope examination. Microbiology examination in vitreous must be taken upon these eyes that the inflammation have last over two weeks in anterior segment. Both degree of PVR and the bleeding indexes in vitreous were estimated by direct funduscopy examination. RESULTS: Both the bleeding indexes and degree of PVR in vitreous in defibrase combined with vancomycin group were lower than both phosphate buffered saline group （P<0.01；P<0.01） and vancomycin group（P<0.05；P<001）；There were 3 eyes (30%) suffered endophthalmitis in both phosphate buffered saline group and defibrase group respectively; There was no eye suffered endophthalmitis in both vancomycin group and defibrase combined with vancomycin group. CONCLUSION: The therapeutics of intravitreal defibrase combined with vancomycin injection can promotes the absorption of vitreous hemorrhage and decrease both the occurrence of bacterial endophthalmitis and the degree of PVR .