AIM:To investigate the expression of VEGF-C,VEGFR-3 and lymphangion genesis in rat cornea after alkali burn. ·METHODS:The rat alkali burnt corneal model were made. The expression of VEGF-C,VEGFR-3 and lymphangion genesis labeled with LYVE-1 in rat cornea were detected by immunohistochemical assay at day 0 and day 1,3,5,7,14,21,28 after burn. In addition,the ultrastructure of lymphangion genesis in rat cornea was observed by electron microscope. ·RESULTS:The expression of VEGF-C and VEGFR-3 in rat cornea were increased after alkali burn. LYVE-1 were detected in rat cornea 3 days after alkali burn.·CONCLUSION:There are some new lymphangion genesis in rat cornea after alkali burn. And meanwhile,the expression of VEGF-C and VEGFR-3 in rat cornea was significantly increased.