AIM:To investigate the effect of multilayer amniotic membrane transplantation in treatment of corneal perforation.·METHODS:The clinical data of 106 patients 118 eyes with corneal perforation who underwent multilayer amniotic membrane transplantation with cryopreserved amniotic membrane materials were retrospectively analyzed. The patients were followed up for 6 to 18 months with an average of 12 months. Healing of corneal perforation,amnion,postoperative best-corrected visual acuity,intraocular pressure and complications were observed in the follow-up.·RESULTS:After 10 days to 3 months postoperatively,all patients were with healed corneal perforation; inner amniotic membranes were firmly with the corneal healing,and they were off the cornea or dissolved and absorbed 10-21 days after the operation in 34 eyes,within 2 months after the operation in 37 eyes,within 3 months after the operation in 47 eyes; after 6 months postoperatively,best-corrected visual acuity:light perception to hand movement in 26 eyes,finger count to 0.05 in 31 eyes,0.06-0.3 in 39 eyes,0.4-0.6 in 10 eyes,0.7-0.8 in 7 eyes,≥0.9 in 5 eyes,compared with preoperative ones,the visual acuity was significantly improved,the difference was significant (χ2=50.68,P<0.01); after 12 months postoperatively,corneal transparency was in 2 eyes,corneal leucoma in 38 eyes,corneal macula in 53 eyes and corneal nebula in 25 eyes; some of those patients who were combined with cataract preoperatively or with corneal leucoma postoperatively underwent cataract surgery or optical penetrating keratoplasty in selected period.·CONCLUSION:The effect of multilayer amniotic membrane transplantation in the treatment of corneal perforation due to various reasons with cryopreserved amniotic membrane,especially the range of corneal perforation within 3mm,is very significant,which can effectively promote the healing process,significantly improve visual acuity,and create favorable conditions for the follow-up treatment.