AIM:To investigate the influence of CTGF-ASON on expression of CTGF and α-SMA induced by transforming growth factor-β1(TGF-β1) in normal human lens epithelial cells(HLECs).METHODS:CTGF-ASON was transduced into normal HLECs by cotransfection with lipofectamine 2000 or direct ways.The transfection ratio of FITC-labeled ASON was measured by fluoroscope and the proliferation of HLECs was measured by CCK-8 assay.The expression of CTGF and α-SMA was detected by RT-PCR.RESULTS:Direct ASON transfection was slow,and no obvious cytotoxicity was observed within 72 hours.The proliferation of HLECs was not inhibited by direct ASON transfection until 72 hours.ASON treated for 48 hours,Culture in TGF-β1 caused profound increase of CTGF and α-SMA that can be inhibited by direct ASON transfection,but not missense oligonucleotides transfection.CONCLUSION:CTGF-ASON can abrogate the influence of TGF-β1 on increase of CTGF and α-SMA mRNA in HLECs.CTGF may be a potential therapeutic target to posterior capsular opacification.