·AIM:To investigate the expression of connective tissue growth factor（CTGF）in rabbits deep sclerectomy（DS）surgery.·METHODS:Human umbilical vein（HUV）was treated with freeze-drying for 6 hours and ⁶⁰Co200cGy irradiation for 30 seconds.The samples were stored in RT.28 rabbits were used,24 of which were performed DS surgery in both eyes.Then one eye was randomly selected to plant HUV according to self-control rule,which composed experimental group.The other eye of the 24 rabbits composed control group.8 rabbits were sacrificed 3,7 and 14 days after operation,respectively.The 8 eyes of the other 4 rabbits were used as normal control.Semi-quantitative RT-PCR and immunohistochemistry were used to assess the expression of CTGF mRNA and protein of deep sclera.·RESULTS:The levels of CTGFmRNA and protein expression in the normal control tissue were lower,but at day 3 they increased more quickly than the control and rapidly peaked at day 7,then decreased at day 14（P<0.01）;there were no significant differences at same time points between experimental group and control group（P>0.05）.·CONCLUSION:These indicated that DS surgery with huaman freeze-dried allograft umbilical vein implant is safe and effective.It has no evident effect of anti-fiber proliferation.The mechanism for its maintanence of decompression chamber may be the result of mechanical water spreading.·