·AIM:To determine iodine concentration of the rabbit vitreous body in the different time after prolonium iodide intramuscular injection,in order to study its permeability through blood-ocular barrier.·METHODS:Fifty adult rabbits were randomly divided into 2 groups.Ⅰgroup was the normal control group;in the Ⅱ group,every rabbit was injected 24mg/kg prolonium iodide with intramuscular injection;After using the drug,every group was determined iodine concentration of vitreous body using Arsenic and Cerium catalysis spectrophotometry in different time.·RESULTS:Iodine content of vitreous body in the intramuscular injection groups was obviously different from that in the normal control group.3 hours after intramuscular injection,iodine concentration in the rabbit vitreous body got to peak value and then began to decline,it was still higher than normal level after 24 hours.·CONCLUSION:Prolonium iodide can enter eye through blood-ocular barrier and metabolize slow through intramuscular injection.·