目的:通过对正常人与不同阶段的原发性开角型青光眼患者的氧化应激状况与GDx-VCC的检查进行对比研究,来探讨氧化应激反应在原发性开角型青光眼发病机制中的重要作用。方法:分别对30例60眼正常人、40例50眼早期青光眼和40例60眼中晚期的开角型青光眼患者的血清进行MDA,CAT,VC,SOD,GSH-Px,GST检测并应用GDx-VCC对其RNFL进行检测,分别比较其RNFL参数的不同。最后比较氧化应激指标与GDx-VCC在不同时期的原发性开角型青光眼患者中的相关性。结果:与正常人群相比,早期原发性开角型青光眼患者血清中GST和MDA即有所升高,中晚期患者升高较为明显,而CAT,GSH-Px,SOD,VC早期即有所降低,中晚期则明显降低;早期原发性开角型青光眼患者的RNFL变薄,中晚期患者的RNFL较早期的明显变薄。两种方法所得的各参数均有极显著统计学差异(P<0.01),具有良好地区分正常人和早期原发性开角型青光眼的能力,对于青光眼的分期诊断中具有一致性。结论:氧化应激的检测可作为临床原发性开角型青光眼的辅助检测手段之一。

AIM:To investigate the important role oxidative stress response plays in the pathogenesis of primary open angle glaucoma through comparative studies of oxidative stress and GDx-VCC checking between the normal and primary open angle glaucoma patients of different stages.METHODS: Totally 30 cases (60 eyes) with normal subjects, 40 patients (50 eyes) with early glaucoma and 40 patients (60 eyes) with advanced open angle glaucoma were detected with serum MDA, CAT, Vc, SOD, GSH-PX, GST detection and RNFL detection by application of GDx-VCC, the difference of RNFL parameters were compared. Finally, the relevance of indicators of oxidative stress and GDx-VCC in primary open angle glaucoma patients at different times were compared.RESULTS: Compared with the normal population, serum GST and MDA of early primary open angle glaucoma patients increased ,that of advanced patients increased more significantly, while the CAT, GSH-PX, SOD, Vc decreased at early stage, and were significantly lower late; RNFL of early primary open angle glaucoma patients became thinner, RNFL in advanced patients became significantly thinner than earlier. The parameters from both methods were statistically significant differences (P<0.01), with good separating capacity between normal and early primary open angle glaucoma, and with consistency for the staging of glaucoma.CONCLUSION:Oxidative stress may be clinically used as auxiliary means of detection for primary open angle glaucoma.

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