AIM:To find special staining methods which is fast, accurate and convenient operation for fungal keratitis tissue section.METHODS: Specimens of 50 cases with the clinical diagnosis of suspected fungal keratitis or corneal ulcer were selected. They were conventionally fixed, dehydrated and dipped in wax for 4μm paraffin sections, then subject to conventional dewaxing to water. Periodic acid-Schiff stain and gomori methenamine silver stain were compaired.RESULTS: Positive detection rate of periodic acid-Schiff stain was 84%, positive detection rate of gomori methenamine silver stain was 79%. Result was statistically significant (P<0.05). CONCLUSION: Periodic acid-Schiff staining method is simple, fast, accurate, high positive rate and lower cost. It is superior to gomori methenamine silver staining, and worth promoting