AIM:To evaluate the therapeutic effectiveness of probing of lacrimal passage in the treatment of the neonatal dacrystitis. METHODS: Forty cases (56 eyes) with neonatal dacryocystitis which had no effect by routine dacryocyst massage with local antibiotics ophthalmic solutions were treated with probing of lacrimal passage under local anesthesia at 6 months of age. Patients were routinely probed for one time and follow-uped averagely for 1 year to observe the therapeutic effect and related factors. RESULTS: Fifty-three eyes(94.6%) in 56 eyes were cured by probing of the lacrimal passage. The success rate for children at 6 months of age was 100% and it showed a downtrend with age.CONCLUSION: Treatment of neonatal dacryocystitis should be taken as early as possible. Probing of lacrimal passage can be performed under local anesthesia and it is simplicity of operation. The tissue damage is slight and the therapeutic effect is obvious. So it is worth being spread.