AIM:To explore the safety,feasibility of paracentesis with Healon agent in glaucoma with persistent high intraocular pressure(IOP).METHODS:A total of 32 cases(34 eyes) of IOP controlled bad of glaucoma underwent intraoperative and preoperative anterior chamber puncture combined with intraoperative application of Healon agent.Visual acuity,IOP,intraoperative and postoperative complications were observed.RESULTS:The operation in all cases were successful and no case occurred with the fulminant hemorrhage in choroidal space.1 case occurred with anterior chamber bleeding,which was absorbed very quickly after therapy,1 case occurred with malignant glaucoma who turned to superior hospital treatment.The visual acuity in a month after operation improved obviously in 18 eyes(53%),remained unchanged in 10 eyes(29%)and decreased in 6 eyes(18%).In a month after operation,there were 30 eyes(88%)in which the IOP was controlled successfully.Three eyes had IOP over 21mmHg,of which 2 eyes were controlled in a month after operation by drugs and local massage.CONCLUSION:The paracentesis of anterior chamber with Healon agent in glaucoma with persistent high IOP can reduce the complications and improve the success rate.