AIM:To research the factors that may influence retinal nerve fibre layer(RNFL) thickness of Leber’s hereditary optic neuropathy(LHON),such as time,visual acuity,age,etc. METHODS:This is a case control study, Zeiss Cirrus 3D optical coherence tomography(OCT, Optic Disc Cube 200×200 program) were used to detect RNFL thickness of peri-disc and each quadrant retina of 64 LHON patients and 88 normal people of same age, data were collected and assorted by patients’ age, gender, visual acuity, how long time after diseases onset, etc. the differences between each group of data were also compared and their clinical value discussed. RESULTS: RNFL thickness of normal people were differed by their age, of which the 18-29 age group was the thickest, younger or elder people both had a thicker RNFL thickness. RNFL thickness of patients with a disease time short of 3 months were thicker than normal people, but patients with a disease time about 3-6 months were almost the same（P=0.745） as normal people, while patients with a disease time longer than 6 months had a much thicker RNFL thickness than normal people. Further more, the change of RNFL thickness in each quadrant was not equal. It was also found that there was no evidence to connect patient’s visual acuity with RNFL thickness in our research（P=0.58）. CONCLUSION: RNFL thickness of LHON patients can be influenced by many factors, such as age and time, but itˊs still lack of evidence to uncover the relationship of RNFL thickness and visual acuity. We believe that OCT can be used to evaluate the progress of LHON and other clinical features.