AIM: To compare the differences between non-contact tonometer (NCT) and Goldmann applanation tonometer (GAT) in the measurement of intraocular pressure (IOP) with different fixation to evaluate the accuracy of NCT on patients with eccentric fixation. METHODS: The IOP of 54 cases (108 eyes)with centric fixation and 49 cases (61 eyes) with eccentric fixation was measured with both NCT and GAT separately. RESULTS: There was no significant difference between IOP measured by NCT (21.21mmHg) and GAT (21.30mmHg) in the centric fixation group. In the eccentric fixation group, IOP measured by NCT (25.33mmHg) was higher than that measured by GAT (21.10mmHg), the difference between the two types of tonometer was positively related to the IOP measured by GAT, the correlated coefficient was 0.792 (P<0.05). CONCLUSION: The NCT overestimated the IOP of patients with eccentric fixation. Examining the fixation nature is helpful to appropriately evaluate IOP measured by NCT, GAT is applied to correct the IOP measured by NCT with eccentric fixation in the clinical work.