AIM:To evaluate the clinical effect of trabeculectomy combined with sclera flap invagination in the treatment of neovascular glaucoma(NVG). METHODS:Forty-eight cases (48 eyes) with neovascularization were performed trabeculectomy combined with sclera flap invagination .The visual acuity, intraocular pressure (IOP), anterior chamber (angle of anterior chamber), hyphema, iris neovascularization and filtering bleb after operation were observed. All patients were followed up for 6-12 months. RESULTS:About 6-12 months after operation, IOP were controlled in less than 21mmHg in 40 cases.Symptoms of 6 cases were alleviated, and the IOP were controlled in less than 21mmHg with treatment of carteolol hydrochloride eye drops. CONCLUSION:Trabeculectomy combined with sclera flap invagination is an ideal treatment for NVG.