AIM:To evaluate the feasibility of application with HRT-Ⅱ to detect morphological alteration of optic disc in primary open angle glaucomatous patients after complex trabeculetomy. METHODS: Totally 20 eyes of 20 cases with different stages of primary open angle glaucoma （POAG）were performed with complex trabeculectomy. All patients IOP were controlled without medicines. The follow-up period was 24 months. HRT-Ⅱscanning was differently undergone in postoperation 3,6,12 and 24 months. RESULTS: There was significant difference (P<0.05) between preoperation and postoperation in cup/disk area ratio (C/D), rim area (RA), mean and superior /inferior retinal nerve fibre layer (mRNFL) in all patients with different stages. CONCLUSION:HRT-Ⅱ can be applied to detect the post-operative morphological alteration of optic disc in POAG patients with high objectivity and repeatability.