AIM:To analyze the outcome and complication of the norvancomycin integrity treatment in bacterial endophthalmitis. METHODS: Twenty-seven patients 27 eyes were observed from July, 2009 to August, 2011. All the patients underwent corpus vitreum bacterial culture and suspecptibility. The vitreous injections of norvancomycin were performed. The treatment also included intravenous injection of norvancomycin and levofloxacin. The patients without improvement underwent vitrectomy with norvancomycin perfusion fluid after two to four days. The best-correct visual acuity, intraocular pressure, anterior chamber and vitreum changes were observed before and after operation. The data were evaluated by statistical analysis. RESULTS:Twenty-seven patients were healing well with increase of visual acuity. 56% specimens were found positive by 67% for gram-positive bacteria. Most gram-positive bacteria were susceptible to norvancomycin while the gram-negative to levofloxacin. 8 patients healed by vitreous injections and 18 patients by vitrectomy. The best correct visual acuity and symptoms were increased. CONCLUSION: The bacterial culture of vitreous could guide the clinical treatment. The norvancomycin integrated treatment is effective for bacterial endophthalmitis.