AIM:To evaluate the clinical effect of nonperforating trabecular surgery (NPTS) combined with amniotic membrane implantation processed with mitomycin for the treatment of primary open angle glaucoma(POAG). METHODS:Fourty cases (58 eyes) with POAG executed NPTS with amniotic membrane implantation processed with mitomycin. Visual acuity, intraocular pressure and the type of blebs were recorded. RESULTS:The intraocular pressure is good after the surgery, there was no statistically significant difference in the visual acuity. The preoperative intraocular pressure was 35.3±6.8mmHg, but postoperative was 17.6±45mmHg, the difference was significant （t=16.531，P<005）. During the visit, 27 eyes （46.55%） had functional blebs, 5 eyes treated with medicine. 9 eyes（15.52%） had blebs with etiolation and presented anemia. CONCLUSION: NPTS with amniotic membrane implantation processed with mitomycin is good for the treatment of POAG.