AIM:To observe the influence of drug-containing plasma on the lacrimal gland epithelial cells and the expression of Bax mRNA and Bcl-2 mRNA in Lacrimal gland epithelial cells.

METHODS:Randomly divided the lacrimal gland epithelial cells in good condition into the flavonoid treatment group without androgen cultural(hereinafter referred to as Buddleia flavonoids group), the control group with androgen cultural(hereinafter referred to as androgen group), and the blank group without androgen cultural(hereinafter referred to as the blank group). Total flavonoids with Buddleia plasma, testosterone propionate and blank plasmain were added to begin the interposal. Then, cell models of dry eye syndrome were established. Trizol reagent was used to extract the whole cell RNA. RT-PCR method was used to detect the expression of the Bax mRNA and Bcl-2 mRNA in lacrimal gland epithelial cells, and the results were contrasted.

RESULTS:The Buddleja group and the androgen group inhibited the expression of Bax mRNA in lacrimal gland epithelial cells, compared with the blank group the difference was significant(P <0.01), the expression of Bax mRNA in the Buddleia group is lower than that in the androgen group, the difference was significant(P <0.01), however, the expression of Bcl-2 mRNA in the Buddleia group and the androgen group were higher than that in the blank group, the difference was significant(P <0.01). The expression of Bcl-2 mRNA in Buddleia group is higher than the androgen group, the difference was significant(P <0.01).

CONCLUSION:Buddleja role of flavonoids in the drug- containing plasma androgen levels decrease in cell models of dry eye syndrome can inhibit the expression of Bax mRNA, decrease the amount of the expression of Bax mRNA, and promote the expression of Bcl-2 mRNA, increase the expression of Bcl-2 mRNA. It is possible that the Buddleja flavonoids drug-containing plasma achieve the inhibition of apoptosis by inhibiting the expression of the Bax mRNA and promoting that of Bcl-2 mRNA in lacrimal gland epithelial cells.