AIM: To study the mechanism of SiPc(C₃₅H₂₇O₅)₈ mediated PDT on retinal pigment epithelial cell(RPE).

METHODS: hRPE cells were divided into blank control group, laser group, photosensitizer SiPc(C₃₅H₂₇O₅)₈ group, PDT 1J group, PDT 2J group. CCK-8, fluorescent microscopes blue excitation were applied in the experimental groups, and active oxygen level of RPE cell mitochondria and RPE cell apoptosis rate were detected by DCFH-DA.

RESULTS: Different concentration photosensitizer \〖SiPc(C₃₅H₂₇O₅)₈\〗 mediated PDT on RPE had different effects, in which 5 μg/L of concentration had the most serious damage.

CONCLUSION:New photosensitizer SiPc(C₃₅H₂₇O₅)₈ mediated PDT on RPE has certain damage effect, its possible mechanism is that the increasing RPE cell mitochondria reactive oxygen species level caused by photosensitizer \〖SiPc(C₃₅H₂₇O₅)₈\〗 mediated PDT results in cell apoptosis.