AIM:To study the effects of different concentrations of sodium ferulate(SF)on human retinal capillary endothelial cells(HRCEC)proliferation and vascular endothelial growth factor(VEGF)expressing with different concentrations in vitro conditions of high glucose.

METHODS: The extracted from corneal transplantation HRSEC and cultured in vitro, which being good growth and at the 3- 4^th generation were taken into experiment. The experimental were divided into blank control group, high glucose control, low glucose control group, high glucose control group,high glucose+different concentrations of SF(1mg/L,2mg/L,4mg/L)group. The result of SF with different concentrations on the proliferation of HRCEC for 48 hours was surveyed by MTT assay. The immunocytochemical method was used to detect low glucose control group, high glucose group and different concentrations of SF(1mg/L, 2mg/L, 4mg/L)group HRCEC of VEGF expression.

RESULTS: The results of MTT colorimetric method showed that: different concentrations of SF in primary cultured HRCEC for 48 hours, cell proliferation inhibition rates were 46.97%, 61.55%, 76.91% and 83.47%. Within a certain range of concentrations of SF can restrain the proliferation of HRCEC in high glucose environment(P<0.05), and in 48 hours was concentration dependent. Low glucose control group compared with the high glucose control group the differences were not statistically significant(P=0.067>0.05). Low glucose control group, high glucose group and SF in concentrations of 1mg/L, 2mg/L, 4mg/L in HRCEC for 48 hours, immunocytochemistry of background gray values with positive gray value difference were respectively 28.27±1.62, 93.67±0.81, 72.67±2.89, 53.73±1.70, 30.93±3.72. Compared to the high glucose control group, expression of VEGF was decreased significantly in experimental groups after 48 hours, the difference was statically significant(P<0.05), in a dose-dependent manner. Compared to the low sugar glucose, VEGF in high glucose control group expression was significantly increased(P<0.05).

CONCLUSION: SF can reduce the HRCEC proliferation and suppress the expression of VEGF in high glucose environment which were cultured in vitro.