Abstract:AIM: To observe the relationship between Prol2Ala polymorphism of peroxisome proliferator activated receptor-γ2(PPAR-γ2)gene and diabetic retinopathy(DR)with type 2 diabetic mellitus(T2DM)of the Han nationality in Shanxi province.
METHODS: Totally 90 patients with T2DM were selected into our research, who were at the age of 40 to 70 years old, diabetic duration from 10 to 20 years, blood pressure <140/90mmHg, and without diabetic nephropathy. Thirty persons were respectively divided into non-DR(NDR)group, nonproliferative-DR(BDR)group and proliferative-DR(PDR)group according to the result of ophthalmology examination. The genotypes of Pro12Ala polymorphism of PPAR-γ2 gene were determined by polymerase chain reaction-restriction fragment length polymorphisms(PCR-RFLP)assay in all the patients.
RESULTS: PCR results showed that there were 2 alleles and 3 genotypes in the groups. The frequency of genotype PP, PA, AA were 40.0%, 53.3%, 6.7% in NDR group, 70.0%, 30.0%, 0.0% in BDR group, 76.7%, 23.3%, 0% in PDR group, respectively. The allele frequency(χ2=10.208)and gene frequency(χ2=10.351)were statistically significant(P<0.05)in these groups. Logistic regression analysis showed that Prol2Ala polymorphism was a dangerous factor in the incidence of DR.
CONCLUSION: The alanine variant of Prol2Ala polymorphism of PPAR-γ2 gene is associated with DR in type 2 diabetes among the Hans in Shanxi area, and the Ala allele might be a protective factor for the development of diabetic retinopathy.
