Amblyopia is a kind of commonly encountered disease. The research method of amblyopia is associated with various clinical and basic subjects. Optical coherence tomography(OCT)is an important ophthalmic examination technique. It is an objective and easy-to-operate means to assess the structure of retina. In recent years, OCT has been frequently used in the retinal examination in amblyopia. This application of OCT in amblyopia is reviewed in this article.