Abstract:AIM:To investigate the role of mitochondrial membrane potential(△ψm)and Caspase 3 in the ACC-2 cell apoptosis induced by As2O3.
METHODS:ACC-2 cells were cultured. The As2O3 of different drug concentration gradients(0, 1.0, 2.0, 4.0, 8.0μmol/L)were applied to ACC-2 cells respectively. The changes in △ψm of ACC-2 cells before and after As2O3's inducing(8.0μmol/L for 24h)were detected by flow cytometry with Rh123 staining. Caspase 3 activity was detected by the multifunctional microplate reader.
RESULTS: Rh123 fluorescence intensity in ACC-2 cells was strongest in the control group, while it weakened in ACC-2 cells in 8.0μmol/L As2O3 treatment group. The difference between two groups was significant(P<0.05). With the increase of As2O3 concentration(0, 1.0, 2.0, 4.0, 8.0μmol/L), Caspase 3 enzyme activity unit in ACC-2 cells gradually increased.
CONCLUSION: As2O3 can induce apoptosis of ACC-2 cells by reducing △ψm. Caspase 3 enzyme activity unit of ACC-2 cells gradually increases with As2O3 concentration increases, which results in activation of the expression of Caspase 3, and the cells' irreversible apoptosis process coming immediately.
