目的：通过腹腔注射葡萄糖的方法建立血糖波动动物模型，以模拟糖尿病患者的血糖波动情形，为在体研究血糖波动的损伤机制提供实验基础。

方法：SD大鼠随机分为四组：正常对照组(NC)、正常波动组(NF)、糖尿病模型组(DM)和糖尿病波动组(DF)。首先腹腔注射STZ诱导糖尿病模型，成模后NF和DF两组大鼠每天3次腹腔注射一定量的葡萄糖造成血糖波动，每2wk进行一次全天血糖检测，根据血糖值绘制“时间-血糖浓度”曲线，观察血糖波动的情况。并计算平均血糖水平(MBG)、平均血糖水平标准差(SDBG)、最大血糖波动幅度(LAGE)和Sclichtkrull Mz值(M值)，从四个不同方面来评价血糖的稳定性。

结果： NC组大鼠全天血糖在正常范围内，平稳无波动，NF组大鼠血糖在5～10mmol/L范围内波动，DM和DF组大鼠血糖维持在较高水平(>20mmol/L)，DM组血糖波动不大，DF组血糖波动明显； NF和DF两组大鼠的血糖变化明显而且规律，波动曲线比较稳定。NF、DM与DF三组大鼠评价血糖稳定性的各项指标均较NC组有明显的增高，DF组升高更显著，多组及两组间比较差异均有统计学意义(P<0.01)。

结论：通过人为腹腔注射葡萄糖的方法能形成明显的血糖波动，可用来模拟糖尿病患者的血糖波动情形，此方法操作简单、损伤小、可重复性好，为在体研究血糖波动的损伤机制以及糖尿病并发症的发病机制提供了实验基础。

AIM: To provide the experimental basis for the in vivo study of blood glucose fluctuation injury mechanism, through intraperitoneal injection of glucose to establish blood glucose fluctuation animal models and to simulate blood glucose fluctuation of patients with diabetes.

METHODS: Rats were randomly divided into four groups: normal control group(NC), normal fluctuation group(NF), diabetes mellitus group(DM)and diabetes fluctuation group(DF). Diabetic models were induced through intraperitoneal injection of STZ. A certain amount of glucose was injected in the rats of group NF and DF intraperitoneally three times a day after the model was established, thereby causing blood glucose fluctuations. Full-day blood glucose monitoring was conducted once every two weeks to observe the blood glucose fluctuation situation, according to blood glucose levels drawing the “time-blood glucose concentration” curve. The stability of blood glucose was evaluated from four different aspects, through calculating mean blood glucose(MBG), standard deviation blood glucose(SDBG), largest amplitude of glycemic excursions(LAGE)and Sclichtkrull Mz value.

RESULTS: The full-day blood glucose of rats in the group NC was in a normal range, and it was stable without fluctuation. The blood glucose of rats in group NF was fluctuated between 5-10mmol/L. The blood glucose of rats in group DM were maintained at higher level(>20mmol/L), without great fluctuation extent and DF with sighificant fluctuation. The blood glucose fluctuation in graoup DF was significant. The blood glucose changes of rats in the group NF and DF were significant and regular. The curve of blood glucose fluctuations was relatively stable. All values of rats in group NF, DM and DF were significantly increased compared with group NC. Group DF was increased more significantly, and the comparison with group NF and DM had significant difference(P<0.01).

CONCLUSION: Significant blood glucose fluctuation can be formed through the method of manual intraperitoneal injection of glucose. The method can be used for simulating the blood glucose fluctuation situation of patients with diabetics. This method has the advantages of simple operation, low injury and good repeatability. It can provide experimental basis for in vivo study of blood glucose fluctuation injury mechanism and pathogenesis of diabetic complications.