Nerve growth factor(NGF)is one of the most important active protein acting on the nervous system, it is applicated widely in ophthalmology, the administration route includes systemic drug administration and local drug administration. Compared to systemic drug administration, local drug administration is targeted, it is more effective to achieve the necessary concentration in eyes and it reduces the risk of side effects. Local drug administration has been a common treatment method in eye diseases. With the development of administration route, corresponding pharmacokinetic researches have received the concern. This review provides a reference for the study on ophthalmological administration routes and pharmacokinetics of nerve growth factor.