Abstract:AIM:To explore the suitable conditions in rapid model of corneal neovascularization(CNV)after thermal burn under different constant temperature in rabbit.
METHODS: Total 45 New Zealand white rabbits were divided randomly into five groups(A, B, C, D, E). A groups: 100℃(n=10), B groups: 200℃(n=10), C groups: 300℃(n=10), D groups: 400℃(n=10), and E groups: control group(n=5). All left eyes of rabbits in A,B,C,D groups were induced corneal neovascularization by constant temperature burning device. The growth of CNV was observed by slit lamp microscope and the area of CNV were recorded on 4 th, 7 th, 14th, 30th days postoperatively. SPSS 19.0 statistical package was used for data analysis, and the data was recorded by mean±standard deviation. Comparison by analysis of variance was made by repeated measures in the area of neovascularization at each time point in groups. Statistical tests were considered significantly when P values were less than 0.05.
RESULTS: On postoperative 4th, 7th, 14th, 30th days: no neovascularization was found after corneal thermal burn in A group, but only a few nebula left(n=2); the area of CNV were(9.16±1.45)mm2,(37.73±5.49)mm2,(62.44±7.54)mm2,(40.28±7.39)mm2 in B group respectively; and(11.45±1.04)mm2,(44.51±4.64)mm2,(66.13±4.13)mm2,(43.04±2.33)mm2 in C group respectively; and(13.23±0.86)mm2,(47.26±4.59)mm2,(67.57±4.56)mm2,(45.59±4.44)mm2 in D group respectively, and part corneal carbide(n=4)was observed as well as corneal perforation(n=6)were found on 3d in D group. No neovascularization was found in normal control group. Comparison of the areas of CNV at each time point between groups was statistically different, P<0.05. Statistical differences were found among B, C, D groups, P<0.05.
COCLUSION: In 4 to 7d, the higher the temperature is, the more the neovascularization area of CNV are. It has no significant difference in 14 to 30d. But corneal carbide and corneal perforation are often found in 400℃ group, so its modeling failure rate is high. It is between 200℃ and 300℃ that repeatability and uniformity of the corneal neovascularization model of rabbit are superior.
