Corneal neovascularization(CNV)is the extensive growth of blood vessels from conjunctiva into cornea. Abnormal angiogenesis plays an important role in the process of CNV, which may result from corneal wound and self-healing. The pathologic growth of blood vessels blocks light, promotes scar formation and causes inflammation, which impairs visual acuity. It is a sight-threatening condition that can decreases eyesight and even leads to blindness. Neovascular eye disease is one of the most common eye diseases in clinical admissions. So, further mechanistic studies are the key to the prevention and treatment of CNV. Determination on the CNV animal models is essential in eye diseases researches. Several main methods of CNV models establishment are summarized in this review.