Abstract:AIM:To investigate the expression of Livin and Caspase-3 proteins in ocular adnexal lymphoproliferative lesions and its clinical significance.
METHODS:Patients suffering from B-cell non-Hodgkin lymphoma(NHL)(n=40)and reactive lymphoid tissue hyperplasia(RLH)(n=10)of ocular adnexal from June 1995 to June 2015 in Qingdao Affiliated Hospital were observed and the 50 paraffin sections were collected. RLH sections were selected to the control group. The patients' age, gender, pathogenic site and pathological types were selected as the classification criteria. The expression of Livin and Caspase-3 proteins were detected by immunohistochemistry and the positive expression rate between lymphomas and RLH was compared. Spearman rank correlation was used to estimate the relationbetween Livin and Caspase-3 in ocular adnexal lymphoma.
RESULTS:The expression of Caspase-3 in B-cell NHL was lower than that in lymphadenosis(P<0.05). While the expression of Livin in NHL was higher(P<0.05). The two proteins had no relation with the age, pathogenic site or gender. But they were related to pathologic type. Livin was highly increased in plasmacytoma(PL)and diffused large B-cell lymphoma(DLBCL)compared with mucosa-associated lymphoid tissue(MALT)lymphoma(P<0.05). While Caspase-3 in PL and DLBCL were lower than that in MALT lymphoma(P<0.05). There was a negative correlation between Livin and Caspase-3 in MALT(r=-0.491,χ2=7.519,P<0.05).
CONCLUSION:Over expression of Livin and low expression of Caspase-3 may play a significant role in the occurrence, development and different pathologic type of ocular lymphoma. The expression of Livin and Caspase-3 combined with each other in NHL. Combined examination of two proteins may be a valuable marker to predict the presence and differentiate the pathologic type of ocular adnexal lymphomas.
