方法：选取我院2014-01/2015-06收治的难治性青光眼患者110例122眼，采用随机数字表法分为观察组55例62眼和对照组55例60眼，均行复合式小梁切除手术治疗，观察组患者术中加用丝裂霉素C。术后随访12mo，对患者眼压及视力进行测量，术前及术后3mo清晨采集空腹血3 mL，ELISA法其血清维生素B12(VB12)、维生素B6(VB6)、叶酸(FA)及白介素-2(IL-2)，白介素-6(IL-6)水平。

结果：术前，两组眼压比较无显著性差异(P>0.05)，术后1wk～12mo，观察组眼压均显著低于对照组(P<0.01)； 术前，两组视力比较无显著性差异(P>0.05)，术后1wk～12mo，观察组视力均显著高于对照组(P<0.01)； 术前，两组患者VB12、VB6、FA、IL-2、IL-6比较均无显著性差异(P>0.05)，术后3mo，观察组VB12、FA、IL-2、IL-6均显著高于对照组(P<0.05)。

结论：丝裂霉素C联合复合式小梁切除术治疗难治性青光眼可有效控制眼压、提高视力，改善病情，提高难治性青光眼的治疗成功率。

METHODS:One hundred and ten cases(122 eyes)in our hospital from January 2014 to June 2015 were divided into observation group(55 cases with 62 eyes)and control group(55 cases with 60 eyes). All patients were treated with complex trabeculectomy, and patients in observation group were given mitomycin C. Before and at postoperatively 12mo, the intraocular pressure and visual acuity were examined. Before and at postoperatively 3mo, the levels of Vitamin B12(VB12), Vitamin B6(VB6), folic acid(FA), interleukin-2(IL-2)and interleukin-6(IL-6)in peripheral blood were detected by enzyme-linked immunosorbent assay(ELISA).

RESULTS:Preoperatively, there were no significant differences on intraocular pressure, visual acuity, VB12, VB6, FA, IL-2, IL-6 between the two groups(P>0.05). At postoperatively 1wk～12mo, the intraocular pressure of observation group was significantly lower than those of control group(P<0.01), the visual acuity was higher than those of control group(P<0.01); VB12, FA, IL-2 and IL-6 of observation group were significantly higher than those of control group(P<0.05)at postoperatively 3mo.

CONCLUSION:Mitomycin C combined with complex trabeculectomy in treatment of refractory glaucoma could control intraocular pressure, improve visual acuity, and enhance the operation success rate.