AIM:To investigate the effect of mitomycin C combined with complex trabeculectomy in treatment of refractory glaucoma.

METHODS:One hundred and ten cases(122 eyes)in our hospital from January 2014 to June 2015 were divided into observation group(55 cases with 62 eyes)and control group(55 cases with 60 eyes). All patients were treated with complex trabeculectomy, and patients in observation group were given mitomycin C. Before and at postoperatively 12mo, the intraocular pressure and visual acuity were examined. Before and at postoperatively 3mo, the levels of Vitamin B12(VB12), Vitamin B6(VB6), folic acid(FA), interleukin-2(IL-2)and interleukin-6(IL-6)in peripheral blood were detected by enzyme-linked immunosorbent assay(ELISA).

RESULTS:Preoperatively, there were no significant differences on intraocular pressure, visual acuity, VB12, VB6, FA, IL-2, IL-6 between the two groups(P>0.05). At postoperatively 1wk～12mo, the intraocular pressure of observation group was significantly lower than those of control group(P<0.01), the visual acuity was higher than those of control group(P<0.01); VB12, FA, IL-2 and IL-6 of observation group were significantly higher than those of control group(P<0.05)at postoperatively 3mo.

CONCLUSION:Mitomycin C combined with complex trabeculectomy in treatment of refractory glaucoma could control intraocular pressure, improve visual acuity, and enhance the operation success rate.