[关键词]
[摘要]
目的：探讨降钙素受体样受体(calcitonin receptor-like receptor，CRLR)基因单核苷酸多态性(single nucleotide polymorpnism，SNP)与汉族原发性前房角关闭人群的关联性。
方法：以流行病学人群为研究对象，采用病例对照设计。收集江苏省阜宁县流行病学调查中筛查出的原发性前房角关闭(primary angle closure，PAC)患者232例，正常对照306例。血样经DNA提取后采用TaqMan-MGB荧光探针法检测CRLR基因的rs1157699(C/T)位点SNP基因型，比较两组等位基因及基因型频率的分布。
结果：病例组的基因型分布(CC 67.4%，CT 30.0%，TT 2.6%)，对照组的基因型分布(CC 71.3%，CT 27.0%，TT 1.7%)，两组之间差异无统计学意义(P>0.05)。
结论：中国汉族人群的CRLR rs1157699位点SNP与原发性前房角关闭无相关性。
[Key word]
[Abstract]
AIM: To study the association of the single nucleotide polymorphism(SNP)rs1157699 in the calcitonin receptor-like receptor(CRLR)gene with primary angle closure(PAC)in a Han Chinese population.
METHODS: All samples, involved 232 PAC cases and 306 controls, were obtained from an epidemiologic survey conducted in Funing, Jiangsu Province, China. Genotyping were carried out by TaqMan-MGB probe using the real time quantitative polymerase chain reaction system to study the relationship between SNP of rs1157699 in CRLR gene and PAC.
RESULTS: The prevalence of CRLRrs1157699 genotype was 67.4%, 30.0%, 2.6% for CC, CT, TT in cases, and 71.3%, 27.0%, 1.7% in controls respectively. There was no difference between the two groups in the distribution of genotype and allele frequencies of rs1157699(P>0.05).
CONCLUSION: Our results do not support a significant role for rs1157699 in CRLR with PAC.
[中图分类号]
[基金项目]
江苏省卫生厅科技项目(No.H201424)
