AIM: To analyze the effect of human amniotic homogenate extract on corneal neovascularization after corneal alkali burn in the process of pigment epithelium derived factor(PEDF)and vascular endothelial growth factor(VEGF)expression and the effect of corneal neovascularization.

METHODS: Totally 32 patients with corneal alkali burn were selected from June 2015 to June 2016 in Foshan, and were randomly divided into Group A and Group B, with a total of 37 eyes. Group A of 17 cases, with a total of 19 eyes, were treated with 40mg/L human amniotic homogenate extract; Group B(n=15), and 18 eyes, treated with 3g/L prednisolone eye drops. In the treatment of 1, 4, 7, 14, 21 and 28d at different time points, we observed the growth of corneal neovascularization, and detected the expression of PEDF and VEGF during angiogenesis.

RESULTS: Group A of patients in the use of human amniotic homogenate extract after the treatment, the expression level of PEDF was significantly higher than that in Group B(P=0.001), after 28d treatment, the expression level of PEDF reached 0.721±0.314. While patients in Group B the expression level of PEDF was only 0.538±0.253. Two groups had significant difference between the expression level of PEDF(P<0.05). The expression level of VEGF in Group A was lower than in Group B at different time points in the test. After the treatment of 28d patients in the Group A, the expression level of VEGF was 0.152±0.020, in Group B the expression level of VEGF was0.302±0.031. Two groups of patients with VEGF expression level between the differences were statistically significant(P<0.05). The patients number in Group A with corneal neovascularization was significantly lower than that in Group B, the difference was statistically significant(P<0.05).

CONCLUSION: Human amniotic homogenate extract can increase the expression of PEDF in corneal neovascularization after corneal alkali burn, inhibit the expression of VEGF and the proliferation of corneal neovascularization.