AIM: To explore the effect of different concentrations of salidroside on H₂O₂ induced oxidative stress damage in human lens epithelium cells(HLEC).

METHODS: HLEC were cultured and divided into negative control group: cultured in normal cultivation; oxidative damage group: treated with 100μmol/L H₂O₂ for 12h; Salidroside low concentration group: 10μmol/L salidroside treated for 24h and H₂O₂ treated for 12h; Salidroside high concentration group: 100μmol/L salidroside treated for 24h and H₂O₂ treated for 12h. MTT method was applied to observe the effect of salidroside on HLEC survival rate. Morphological change of each group were observed and recorded under inverted microscope. DCFH-DA fluorescent probe was applied to detect intracellular ROS changes; content of malondialdehyde(MDA), superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in supernatants were detected by pectrophotometer.

RESULTS: Salidroside obviously inhibited H₂O₂-induced HLEC vitality decline, inhibited ROS generation in cells, causing SOD, GSH-Px levels increased and MDA levels decreased.

CONCLUSION: Salidroside inhibited H₂O₂ induced HLEC injury by decreasing the intracellular MDA content levels and increasing SOD, GSH-Px content levels, which conclude that salidroside may have a certain role in the treatment of HLEC damage.