目的：观察脂质运载蛋白2(lipocalin-2， LCN-2)对体外培养的小鼠视网膜内皮细胞(retinal vascular endothelial cells， RVECs)增殖、迁移和管腔形成的影响及其在视网膜新生血管中的作用。

方法：取生长状况良好的RVECs分为不同浓度组：分别以0、5、10μmol/L LCN-2作用细胞48h。采用EdU法检测细胞增殖，Transwell法检测细胞迁移，Matrigel法检测管腔形成。

结果：不同浓度LCN-2作用的细胞增殖率大于对照组，差异有统计学意义(P<0.05)，不同浓度LCN-2组RVECs细胞迁移数目多于对照组，差异有统计学意义(P<0.05)，不同浓度LCN-2组细胞管腔形成数明显多于对照组，差异有统计学意义(P<0.05)，且细胞增殖、迁移和管腔形成均随着LCN-2浓度的升高而增加。

结论：LCN-2能够明显促进RVECs的血管生成过程，提示LCN-2是一种重要的促视网膜新生血管形成的因子。

METHODS: Well cultured RVECs were divided to different groups which were treated with 0, 5, 10 μmol/L LCN-2 for 48h, respectively. Cell proliferation, migration and tube formation were detected by using the EDU assay, transwell assay and matrigel assay, respectively.

RESULTS: The cell proliferation rate was promoted in both low and high dose of LCN-2 groups compared to the control cells(P<0.05). The number of migrated cells in both LCN-2 groups was significantly larger than that of the control group(P<0.05). The number of capillary-like tube structures of both LCN-2 groups was significantly larger than that of the control cells(P<0.05). In addition, cell proliferation, migration and tube formation were all increased with the increase of LCN-2 concentration.

CONCLUSION: LCN-2 could obviously promote the angiogenesis capacity of RVECs, suggesting that LCN-2 is an important pro-angiogenic factor in retinal angiogenesis.