石斛酚对高糖诱导的人视网膜微血管内皮细胞的保护作用
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

上海市眼底病重点实验室(No.030105); 上海市卫生和计划生育委员会科研课题(No.201640245)


Protective effect of gigantolon human retinal microvascular endothelial cells induced by high glucose
Author:
Affiliation:

Fund Project:

Shanghai Key Laboratory of Ocular Fundus Diseases(No. 030105); Scientific Research Project of Shanghai Municipal Commission of Health and Family Planning(No. 201640245)

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探讨石斛酚对高糖诱导的人视网膜微血管内皮细胞(HRMECs)的醛糖还原酶(AR)通路及氧化应激的保护作用。

    方法:将体外培养的HRMECs分为5组:正常组(葡萄糖5.5mmol/L)、甘露醇组(葡萄糖5.5mmol/L +甘露醇24.5mmol/L)、高糖组(葡萄糖30mmol/L)、石斛酚组(葡萄糖30mmol/L+石斛酚5μmol/L)、依帕司他组(葡萄糖30mmol/L+依帕司他1μmol/L)。通过MTS实验检测细胞活力,DCFH-DA荧光探针法检测细胞内活性氧(ROS)水平; Western blotting法检测核因子κB(NF-κB)的蛋白表达; qRT-PCR法检测AR、HIF-1α及VEGF的mRNA表达。

    结果:DCFH-DA荧光探针法结果显示石斛酚可明显降低高糖环境下细胞内的ROS水平。与高糖组相比,石斛酚组NF-κB的蛋白表达,AR、HIF-1α及VEGF的mRNA表达也都明显下降。依帕司他组的AR及NF-κB表达趋势与石斛酚组一致。

    结论:石斛酚与依帕司他都可抑制AR/NF-κB通路的激活,同时石斛酚还可抑制高糖诱导的HRMECs氧化应激反应。石斛酚在糖尿病视网膜病变的发生发展中可能有一定的保护作用,可能帮助减少新生血管的生成。

    Abstract:

    AIM: To investigate the protective effects of gigantol on aldose reductase(AR)pathway and oxidative stress induced by high glucose in human retinal microvascular endothelial cells(HRMECs).

    METHODS: HRMECs were cultured and divided into the five groups: normal glucose group, mannitol group,high glucose group, gigantol group and epalrestat group. Cell vitality was detected by the MTS assay, reactive oxygen species(ROS)levels were detected by DCFH-DA fluorescent probe, protein expressions of nuclear factor kappa B(NF-κB)were observed by Western blotting, mRNA levels of AR, hypoxia inducible factor-1α(HIF-1α)and vascular endothelial growth factor(VEGF)were measured by qRT-PCR.

    RESULTS: DCFH-DA fluorescent probe levels showed that gigantol reduced ROS generation obviously, and protein expressions of NF-κB, mRNA expressions of AR,HIF-1α and VEGF were all decreased. The expression trends of AR and NF-κB in epalrestat group were consistent with gigantol group.

    CONCLUSION: Gigantol and epalrestat could inhibit the activation of AR/NF-κB pathway, and gigantol also could inhibit high glucose induced oxidative stress and in HRMECs, which may have a protective effect on diabetic retinopathy(DR), and improve angiogenesis.

    参考文献
    相似文献
    引证文献
引用本文

张梅,李春霞,韦芳,等.石斛酚对高糖诱导的人视网膜微血管内皮细胞的保护作用.国际眼科杂志, 2019,19(2):209-213.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2018-08-06
  • 最后修改日期:2019-01-02
  • 录用日期:
  • 在线发布日期: 2019-01-17
  • 出版日期: