AIM:To explore the effect of amniotic lacrimal stent graft on castrated male rabbits of dry eye.

METHODS: Thirty-six adult male rabbits were divided into two groups. Group A was treated with false castration, group B was pseudo-implantation group, and group C was implanted with lacrimal canal. Fluorescein(FL)staining and scoring and SⅠt examination, and confocal microscopy were performed at 2, 4, 6wk after implantation.

RESULTS: The cornea of group A was smooth and clear, and the FL staining was not obvious. In group B, the cornea was rough, and the corneal epithelium was spotted with stained spots. The corneal surface of group C was smoother and more transparent than that of sham operation group and the punctate staining was not obviously. The FL staining scores and SⅠt examination were significant(P<0.05)at 2, 4, and 6wk after implantation. FL staining and scoring and SⅠt, tear protein determination, confocal microscopy changes in corneal nerve. Confocal microscopy showed that group A was a relatively straight plexus, the amount was large, clearly visible. In the group B, the inferior nerve of the corneal epithelial cells was visible and the number was reduced. The corneal epithelial nerve in group C was relatively straight, and the number was slightly reduced compared with the normal male rabbit corneal epithelial nerve.

CONCLUSION: The amniotic lacrimal duct stent can significantly improve the symptoms and signs of castrated castrated male rabbit dry eye syndrome, and has a therapeutic effect.